Human KIR2DL5 HEK293 Overexpression Lysate


Human KIR2DL5 HEK293 Overexpression Lysate: 产品信息

This Human KIR2DL5 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of KIR2DL5 protein (Cat: 16053-H02H) from the overexpression lysate was verified.
HEK293 Cells
A DNA sequence encoding the human KIR2DL5A (NP_065396.1) (Met1-His240) was expressed with the Fc region of human IgG1 at the C-terminus.
The recombinant human KIR2DL5A consists of 457 amino acids and predicts a molecular mass of 50.3 kDa.

Human KIR2DL5 HEK293 Overexpression Lysate: 使用指南

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human KIR2DL5 HEK293 Overexpression Lysate: 别称

Human CD158F Overexpression Lysate; Human KIR2DL5 Overexpression Lysate; Human KIR2DL5.1 Overexpression Lysate; Human KIR2DL5.3 Overexpression Lysate

KIR2DL5 背景信息

KIR2DL5A (Killer Cell Immunoglobulin Like Receptor, Two Ig Domains And Long Cytoplasmic Tail 5A) is a Protein Coding gene. The encoded protein belongs to the immunoglobulin superfamily. The most recently described member of the KIR family, KIR2DL5, is represented in human populations by at least four gene variants, whose exons differ by two to eight nucleotides. KIR2DL5 (CD158f) is the most recently identified inhibitory member of human killer-cell Ig-like receptors (KIRs), which enable NK cells to sense self-HLA. KIR2DL5 is encoded by two paralogous genes displaying copy number variation and allelic polymorphism-KIR2DL5A and KIR2DL5B. Functional polymorphisms in both its promoter and its coding regions are critical for understanding the KIR2DL5 role in immunity and its importance for human health.
killer cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 5A
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