Human LILRB3 HEK293 Overexpression Lysate

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Human LILRB3 HEK293 Overexpression Lysate: 产品信息

产品描述
This Human LILRB3 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of LILRB3 protein (Cat: 11978-H08H) from the overexpression lysate was verified.
表达宿主
HEK293 Cells
种属
Human
蛋白构建信息
A DNA sequence encoding the human LILRB3 (NP_006855.2) extracellular domain (Met 1-Glu 443) was expressed, with a polyhistidine tag at the C-terminus.
分子量
The recombinant human LILRB3 consists of 431 amino acids and predictes a molecular mass of 47.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh LILRB3 is approximately 55-60 kDa due to glycosylation.

Human LILRB3 HEK293 Overexpression Lysate: 使用指南

制备方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解缓冲液
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
使用建议
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
缓冲液
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
应用
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human LILRB3 HEK293 Overexpression Lysate: 别称

Human CD85A Overexpression Lysate; Human HL9 Overexpression Lysate; Human ILT-5 Overexpression Lysate; Human ILT5 Overexpression Lysate; Human LILRA6 Overexpression Lysate; Human LIR-3 Overexpression Lysate; Human LIR3 Overexpression Lysate; Human MGC138403 Overexpression Lysate; Human PIRB Overexpression Lysate; Human XXbac-BCX105G6.7 Overexpression Lysate

LILRB3 背景信息

Leukocyte immunoglobulin-like receptor subfamily B member 3, also known as Leukocyte immunoglobulin-like receptor 3, Immunoglobulin-like transcript 5, Monocyte inhibitory receptor HL9, CD85 antigen-like family member A, CD85a and LILRB3, is a single-pass type I membrane protein that belongs to the leukocyte receptor cluster (LRC) present on 19q13.4. LILRB3 / CD85a contains four Ig-like C2-type (immunoglobulin-like) domains. LILRB3 / CD85a contains three copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in the modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. LILRB3 / CD85a is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found.
全称
leukocyte immunoglobulin like receptor B3
参考文献
  • Huang,J. et al., 2010, J Virol. 84 (18): 9463-71.
  • Arm J.P. et al., 1997, J. Immunol. 159:2342-2349.
  • Wende H. et al., 2000, Immunogenetics 51:703-713.
  • Yu L.-R. et al., 2007,J. Proteome Res. 6:4150-4162.
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