Human LRP10 HEK293 Overexpression Lysate

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Human LRP10 HEK293 Overexpression Lysate: 产品信息

产品描述
This Human LRP10 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of LRP10 protein (Cat: 13228-H02H) from the overexpression lysate was verified.
表达宿主
HEK293 Cells
种属
Human
蛋白构建信息
A DNA sequence encoding the human LRP10 (Q7Z4F1-1) extracellular domain (Met 1-Lys 440) was fused with the Fc region of human IgG1 at the C-terminus.
分子量
The secreted recombinant human LRP10/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 665 amino acids and has a predicted molecular mass of 73 kDa. The apparent molecular mass of rhLRP10/Fc monomer is approximately 80-90 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Human LRP10 HEK293 Overexpression Lysate: 使用指南

制备方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解缓冲液
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
使用建议
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
缓冲液
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
应用
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human LRP10 HEK293 Overexpression Lysate: 别称

Human LRP9 Overexpression Lysate; Human MST087 Overexpression Lysate; Human MSTP087 Overexpression Lysate

LRP10 背景信息

LRP10 (low-density lipoprotein Receptor Related Protein 10) is a Protein Coding gene. 2 alternatively spliced human isoforms have been reported. This gene encodes a low-density lipoprotein receptor family protein. A similar protein in mouse is thought to play a role in the uptake of apolipoprotein E-containing lipoproteins. Human LRP10 cDNA encodes a 1905 amino acid type I membrane protein consisting of five functional domains characteristic of the LDLR gene family. It is widely expressed in the esophagus, colon, and other tissues. LRP10 plays a significant role in brain physiology other than lipoprotein metabolism. It is associated with a series of neurodegenerative diseases, such as Alzheimer's disease and Parkinson's disease which share genetic risk factors and pathophysiological processes with amyotrophic lateral sclerosis (ALS).
全称
low density lipoprotein receptor-related protein 10
参考文献
  • Jeong YH, et al. (2010) The low-density lipoprotein receptor-related protein 10 is a negative regulator of the canonical Wnt/beta-catenin signaling pathway. Biochem Biophys Res Commun. 392(4): 495-9.
  • Beisiegel U, et al. (1991) Lipoprotein lipase enhances the binding of chylomicrons to low density lipoprotein receptor-related protein. Proc Natl Acad Sci U S A. 88(19): 8342-6.
  • Strickland DK, et al. (1990) Sequence identity between the alpha 2-macroglobulin receptor and low density lipoprotein receptor-related protein suggests that this molecule is a multifunctional receptor. J Biol Chem. 265(29): 17401-4.
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