This Human LRRC3B overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of LRRC3B protein (Cat: 15120-H08H) from the overexpression lysate was verified.
A DNA sequence encoding the human LRRC3B (NP_443185.1) (Met1-Tyr204) was expressed with a polyhistidine tag at the C-terminus.
The recombinant human LRRC3B consists 182 amino acids and predicts a molecular mass of 20.4 kDa.
Human LRRC3B HEK293 Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human LRRC3B HEK293 Overexpression Lysate: 别称
Human LRP15 Overexpression Lysate
LRRC3B expression is downregulated in many kinds of malignant tumors and it is regarded as a tumor inhibition protein. The leucine-rich repeat-containing 3B (LRRC3B) gene is a putative tumor suppressor located on human chromosome 3 in the 3p24 region. LRRC3B is frequently altered in colon and gastric cancers and also in leukemias.LRRC3B expression is downregulated in lung cancer cell lines and LRRC3B could inhibit lung cancer cell proliferation, invasion, and cell cycle progress. LRRC3B could be a new target for lung cancer therapy.LRRC3B depletion in HBE cells promoted proliferation and invasion，suggesting that LRRC3B may serve as an important tumor suppressor in NSCLC.