Human MEGF10 HEK293 Overexpression Lysate

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Human MEGF10 HEK293 Overexpression Lysate: 产品信息

产品描述
This Human MEGF10 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of MEGF10 protein (Cat: 16064-H02H) from the overexpression lysate was verified.
表达宿主
HEK293 Cells
种属
Human
蛋白构建信息
A DNA sequence encoding the human MEGF10 (XP_016865476.1) (Met1-Gly857) was expressed with the Fc region of human IgG1 at the C-terminus.
分子量
The recombinant human MEGF10 consists 843 amino acids and predicts a molecular mass of 90.2 kDa.

Human MEGF10 HEK293 Overexpression Lysate: 使用指南

制备方法
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解缓冲液
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
使用建议
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
缓冲液
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
应用
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human MEGF10 HEK293 Overexpression Lysate: 别称

Human EMARDD Overexpression Lysate

MEGF10 背景信息

MEGF10 (Multiple EGF Like Domains 10) is a Protein Coding gene. 2 alternatively spliced human isoforms have been reported. This gene encodes a member of the multiple epidermal growth factor-like domains protein family. The encoded protein plays a role in cell adhesion, motility, and proliferation, and is a critical mediator of apoptotic cell phagocytosis as well as amyloid-beta peptide uptake in the brain. MEGF10 may also function in the mosaic spacing of specific neuron subtypes in the retina through homotypic retinal neuron repulsion. It belongs to the MEGF family. Diseases associated with MEGF10 include Myopathy, Areflexia, Respiratory Distress, And Dysphagia, Early-Onset, and Dysphagia.
全称
multiple EGF-like-domains 10
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