Human MRPL44 Baculovirus-Insect cells Overexpression Lysate


Human MRPL44 Baculovirus-Insect cells Overexpression Lysate: 产品信息

This Human MRPL44 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of MRPL44 protein (Cat: 15069-H08B) from the overexpression lysate was verified.
Baculovirus-Insect cells
A DNA sequence encoding the human MRPL44 (Q9H9J2) (Met1-Ser332) was expressed with a C-terminal polyhistidine tag.
The secreted recombinant human MRPL44 consists of 312 amino acids and predicts a molecular mass of 35.7 KDa. The apparent molecular mass of the protein is approximately 40 KDa in SDS-PAGE under reducing conditions.

Human MRPL44 Baculovirus-Insect cells Overexpression Lysate: 使用指南

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human MRPL44 Baculovirus-Insect cells Overexpression Lysate: 别称

Human COXPD16 Overexpression Lysate; Human L44MT Overexpression Lysate; Human MRP-L44 Overexpression Lysate

MRPL44 背景信息

MRPL44 (Mitochondrial Ribosomal Protein L44) is a Protein Coding gene. MRPL44 encodes a protein in the large subunit of the mitochondrial ribosome and is suggested to locate near the tunnel exit of the yeast mitochondrial ribosome. It belongs to the mitochondrion-specific ribosomal protein mL44 subfamily. In the patient fibroblasts, decreased MRPL44 affected assembly of the large ribosomal subunit and stability of 16S rRNA leading to complex IV deficiency. It may have a function in the assembly/stability of nascent mitochondrial polypeptides exiting the ribosome. MRPL44 is widely expressed in the bone marrow, lymph node, and other tissues. Diseases associated with MRPL44 include Combined Oxidative Phosphorylation Deficiency 16 and Combined Oxidative Phosphorylation Deficiency 1.
mitochondrial ribosomal protein L44
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