This Human MUCL1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of MUCL1 protein (Cat: 15716-H08H) from the overexpression lysate was verified.
A DNA sequence encoding the human MUCL1 (NP_477521.1) (Met1-Pro90) was expressed with a polyhistidine tag at the C-terminus.
The recombinant human MUCL1 consists 81 amino acids and predicts a molecular mass of 8.4 kDa.
Human MUCL1 HEK293 Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.