Human NOTCH4 Baculovirus-Insect Overexpression Lysate


Human NOTCH4 Baculovirus-Insect Overexpression Lysate: 产品信息

This Human NOTCH4 overexpression lysate was created in Baculovirus-Insect Cells and intented for use as a Western blot (WB) positive control. Purification of NOTCH4 protein (Cat: 11869-H08B) from the overexpression lysate was verified.
Baculovirus-Insect Cells
A DNA sequence encoding the human NOTCH4 (AAH63815.1) (Met1-Phe637) was expressed with a polyhistidine tag at the C-terminus.
The recombinant human NOTCH4 consists 625 amino acids and predicts a molecular mass of 66.1 kDa.

Human NOTCH4 Baculovirus-Insect Overexpression Lysate: 使用指南

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human NOTCH4 Baculovirus-Insect Overexpression Lysate: 别称

Human INT3 Overexpression Lysate

NOTCH4 背景信息

NOTCH4 (Notch Receptor 4) is a Protein Coding gene. Notch4 belongs to a family of transmembrane receptors that play an important role in vascular development and maintenance. The NOTCH4 gene is located at 6p21.3 and is involved in the development and patterning of the central nervous systems. It regulates signaling pathways associated with neuronal maturation, a process involved in the development and patterning of the central nervous system. The NOTCH4 gene has also been identified as a possible susceptibility gene for schizophrenia (SCZ). It is broadly expressed in fat, lung, and other tissues. The NOTCH4 gene, located within the MHC region, is involved in cellular differentiation and has varying effects dependent on tissue type.
notch 4
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