Human NRXN3 HEK293 Overexpression Lysate

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Human NRXN3 HEK293 Overexpression Lysate: 产品信息

产品描述
This Human NRXN3 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of NRXN3 protein (Cat: 11843-H08H) from the overexpression lysate was verified.
表达宿主
HEK293 Cells
种属
Human
蛋白构建信息
A DNA sequence encoding the human NRXN3 beta isoform 2 (NP_620426.2) extracellular domain (Met 1-Thr 357) was expressed, with a polyhistidine tag at the C-terminus.
分子量
The recombinant human NRXN3 consists of 333 amino acids and predictes a molecular mass of 36 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhNRXN3 is approximately 50-60 kDa due to glycosylation.

Human NRXN3 HEK293 Overexpression Lysate: 使用指南

制备方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解缓冲液
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
使用建议
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
缓冲液
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
应用
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human NRXN3 HEK293 Overexpression Lysate: 别称

Human C14orf60 Overexpression Lysate

NRXN3 背景信息

Neurexin-3-beta, also known as Neurexin III-beta and NRXN3, is a single-pass type I membrane protein that belongs to the neurexin family. It contains one laminin G-like domain. It is a neuronal cell surface protein that may be involved in cell recognition and cell adhesion. Neurexins are a family of proteins that function in the vertebrate nervous system as cell adhesion molecules and receptors. They are encoded by several unlinked genes of which two, NRXN1 and NRXN3, are among the largest known human genes. Three of the genes ( NRXN1, NRXN2, NRXN3 ) utilize two alternate promoters and include numerous alternatively spliced exons to generate thousands of distinct mRNA transcripts and protein isoforms. The majority of transcripts are produced from the upstream promoter and encode alpha-neurexin isoforms; a much smaller number of transcripts are produced from the downstream promoter and encode beta-neurexin isoforms. The alpha-neurexins contain EGF-like sequences and laminin G domains and have been shown to interact with neurexophilins. The beta-neurexins lack EGF-like sequences and contain fewer laminin G domains than alpha-neurexins. NRXN3 has been linked to a genetic predisposition towards some conditions such as alcohol or drug addiction, or obesity.
全称
neurexin 3
参考文献
  • Occhi G. et al., 2002, Biochem. Biophys. Res. Commun. 298:151-5.
  • Rowen L, et al., 2002, Genomics. 79 (4): 587-97.
  • Olsen J.V. et al., 2006, Cell 127:635-48.
  • Hishimoto A, et al., 2007, Human Molecular Genetics.16 (23): 2880-91.
  • Oppermann F.S. et al., 2009, Mol. Cell. Proteomics 8:1751-64. 
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