This Human PILRB overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of PILRB protein (Cat: 13746-H07H) from the overexpression lysate was verified.
A DNA sequence encoding the human PILRB (NP_839956.1) (Gln20-Ala189) was expressed with a polyhistidine tag at the N-terminus.
The recombinant human PILRB consists of 189 amino acids and predicts a molecular mass of 21.5 kDa.
Human PILRB HEK293 Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human PILRB HEK293 Overexpression Lysate: 别称
Human FDFACT1 Overexpression Lysate; Human FDFACT2 Overexpression Lysate
paired immunoglobin-like type 2 receptor beta
Wilson MD, et al. (2006) Comparative analysis of the paired immunoglobulin-like receptor (PILR) locus in six mammalian genomes: duplication, conversion, and the birth of new genes. Physiol Genomics. 27 (3): 201-18.
Orr MT, et al. (2010) Natural killer cell education and tolerance. Cell. 142(6): 847-56.
Mousseau DD, et al. (2000) PILRalpha, a novel immunoreceptor tyrosine-based inhibitory motif-bearing protein, recruits SHP-1 upon tyrosine phosphorylation and is paired with the truncated counterpart PILRbeta. J Biol Chem. 275 (6): 4467-74.