This Human PLA2G2A overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of PLA2G2A protein (Cat: 11187-H08H) from the overexpression lysate was verified.
A DNA sequence encoding the human PLA2G2A (NP_000291.1) (Met 1-Cys 144) was expressed, with a polyhistidine tag at the C-terminus.
The recombinant human PLA2G2A consists of 135 amino acids and predictes a molecular mass of 15.4 kDa. In SDS-PAGE under reducing conditions, rh PLA2G2A migrates as an approximately 19 kDa band due to glycosylation.
Human PLA2G2A HEK293 Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human PLA2G2A HEK293 Overexpression Lysate: 别称
Human MOM1 Overexpression Lysate; Human PLA2 Overexpression Lysate; Human PLA2B Overexpression Lysate; Human PLA2L Overexpression Lysate; Human PLA2S Overexpression Lysate; Human PLAS1 Overexpression Lysate; Human sPLA2 Overexpression Lysate
Phospholipase A2, membrane associated, also known as Phosphatidylcholine 2-acylhydrolase 2A, Group IIA phospholipase A2, Non-pancreatic secretory phospholipase A2 and PLA2G2A, is a peripheral membrane protein that belongs to the phospholipase A2 family. PLA2G2A is found in many cells and also extracellularly. The membrane-bound and secreted forms of PLA2G2A are identical. PLA2G2A has been proposed to play a role in anti-bacterial defense, inflammation and eicosanoid generation, in clearance of apoptotic cells, and in the Wnt signaling pathway. PLA2G2A is thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. PLA2G2A catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. PLA2G2A might be a factor in human colorectal tumorigenesis.
phospholipase A2, group IIA (platelets, synovial fluid)
Praml,C. et al., 1998, Oncogene. 17 (15):2009-12.
Fijneman,R.J. et al., 2008,Front Biosci 13 :4144-74.
Fijneman,R.J. et al., 2009, Cell Oncol 31 (5):345-56.