This Human PRELP overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of PRELP protein (Cat: 13538-H04H) from the overexpression lysate was verified.
A DNA sequence encoding the human PRELP (NP_002716.1) (Gln21-Ile382) was expressed with the Fc region of mouse IgG1 at the N-terminus.
The recombinant human PRELP/mFc comprises 598 amino acids and has a predicted molecular mass of 68.3 kDa. The apparent molecular mass of the protein is approximately 80-90 kDa in SDS-PAGE under reducing conditions.
Human PRELP HEK293 Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human PRELP HEK293 Overexpression Lysate: 别称
Human MST161 Overexpression Lysate; Human MSTP161 Overexpression Lysate; Human SLRR2A Overexpression Lysate
PRELP (proline/arginine-rich end leucine-rich repeat protein) is a member of the leucine-rich repeat (LRR) family of extracellular matrix proteins in connective tissue. In contrast with other members of the family, the N-terminal domain of PRELP has a high content of proline and positively charged amino acids. This domain has previously been shown to bind chondrocytes and to inhibit osteoclast differentiation. PRELP is a 58-kDa proteoglycan found in a variety of extracellular matrices, including cartilage and at several basement membranes.
proline/arginine-rich end leucine-rich repeat protein