Human Procalcitonin/CALCA HEK293 Overexpression Lysate


Human Procalcitonin/CALCA HEK293 Overexpression Lysate: 产品信息

This Human Procalcitonin/CALCA overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Procalcitonin/CALCA protein (Cat: 13235-H05H) from the overexpression lysate was verified.
HEK293 Cells
A DNA sequence encoding the human CALCA (NP_001732.1) (Met1-Asn141) was expressed with the Fc region of mouse IgG1 at the C-terminus.
The recombinant human CALCA/mFc comprises 350 amino acids and has a predicted molecular mass of 39.2 kDa. The apparent molecular mass of the monomer is approximately 41.6 and 31.2 kDa in SDS-PAGE under reducing conditions.

Human Procalcitonin/CALCA HEK293 Overexpression Lysate: 使用指南

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human Procalcitonin/CALCA HEK293 Overexpression Lysate: 别称

Human CALC1 Overexpression Lysate; Human CGRP Overexpression Lysate; Human CGRP-I Overexpression Lysate; Human CGRP1 Overexpression Lysate; Human CT Overexpression Lysate; Human KC Overexpression Lysate; Human PCT Overexpression Lysate; Human Procalcitonin Overexpression Lysate

Procalcitonin/CALCA 背景信息

calcitonin-related polypeptide alpha
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后 U.S.A.