Human RP2 Baculovirus-Insect cells Overexpression Lysate


Human RP2 Baculovirus-Insect cells Overexpression Lysate: 产品信息

This Human RP2 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of RP2 protein (Cat: 11092-H09B) from the overexpression lysate was verified.
Baculovirus-Insect cells
A DNA sequence encoding the human XRP2 (NP_008846.2) (Met 1-Thr 350) was fused with the GST tag at the N-terminus.
The recombinant human XRP2/GST chimera consists of 575 amino acids and predicts a molecular mass of 66 kDa as estimated in SDS-PAGE under reducing conditions.

Human RP2 Baculovirus-Insect cells Overexpression Lysate: 使用指南

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human RP2 Baculovirus-Insect cells Overexpression Lysate: 别称

Human DELXp11.3 Overexpression Lysate; Human KIAA0215 Overexpression Lysate; Human NM23-H10 Overexpression Lysate; Human NME10 Overexpression Lysate; Human RP2 Overexpression Lysate; Human TBCCD2 Overexpression Lysate; Human XRP2 Overexpression Lysate

RP2 背景信息

XRP2, also known as Protein XRP2 and RP2, is a member of the TBCC (tubulin cofactor C) family and contains one C-CAP/cofactor C-like domain. This protein is encoded by the RP2 gene in humans. XRP2 stimulates the GTPase activity of tubulin, but does not enhance tubulin heterodimerization. XRP2 acts as guanine nucleotide dissociation inhibitor for ARL3. Defects in RP2 gene are the cause of retinitis pigmentosa type 2 (RP2), also known as X-linked retinitis pigmentosa 2 (XLRP-2). It leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
retinitis pigmentosa 2 (X-linked recessive)
  • Schwahn U., et al.,(1998), Positional cloning of the gene for X-linked retinitis pigmentosa 2. Nat. Genet. 19:327-332.
  • Ross M.T., et al., (2005), The DNA sequence of the human X chromosome.Nature 434:325-337.
  • Vaca Jacome A.S., et al.,(2015), N-terminome analysis of the human mitochondrial proteome. Proteomics 15:2519-2524.
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