Human SERPINB4 Baculovirus-Insect cells Overexpression Lysate

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Human SERPINB4 Baculovirus-Insect cells Overexpression Lysate: 产品信息

产品描述
This Human SERPINB4 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of SERPINB4 protein (Cat: 12990-H07B) from the overexpression lysate was verified.
表达宿主
Baculovirus-Insect cells
种属
Human
蛋白构建信息
A DNA sequence encoding the human SERPINB4 (P48594) (Met 1-Pro 390) was expressed, with a polyhistidine tag at the N-terminus.
分子量
The recombinant human SERPINB4 consists of 409 amino acids and predicts a molecular mass of 47.1 kDa. It migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions.

Human SERPINB4 Baculovirus-Insect cells Overexpression Lysate: 使用指南

制备方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解缓冲液
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
使用建议
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
缓冲液
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
应用
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human SERPINB4 Baculovirus-Insect cells Overexpression Lysate: 别称

Human LEUPIN Overexpression Lysate; Human PI11 Overexpression Lysate; Human SCCA-2 Overexpression Lysate; Human SCCA1 Overexpression Lysate; Human SCCA2 Overexpression Lysate; Human Serpin B4 Overexpression Lysate

SERPINB4 背景信息

Overexpression of SERPINB4 in tumor cells inhibited recombinant GrM-induced as well as NK cell-mediated cell death and this inhibition depended on the reactive center loop of the serpin.
全称
serpin peptidase inhibitor, clade B (ovalbumin), member 4
参考文献
  • de Koning PJ, et al. (2011) Intracellular Serine Protease Inhibitor SERPINB4 Inhibits Granzyme M-Induced Cell Death. PLoS One. 6(8): e22645.
  • Chechlinska M, et al. (2010) Squamous cell carcinoma antigen 1 and 2 expression in cultured normal peripheral blood mononuclear cells and in vulvar squamous cell carcinoma. Tumour Biol. 31(6): 559-67.
  • Shiiba M, et al. (2010) Down-regulated expression of SERPIN genes located on chromosome 18q21 in oral squamous cell carcinomas. Oncol Rep. 24(1): 241-9. en, Der p 1. J Biol Chem. 279(7): 5081-7.
  • Murakami A, et al. (2008) Relationship between decreased expression of squamous cell carcinoma antigen 2 and E-cadherin in primary cervical cancer lesions and lymph node metastasis. Oncol Rep. 19(1): 99-104.
  • Silverman GA, et al. (1998) SCCA1 and SCCA2 are proteinase inhibitors that map to the serpin cluster at 18q21.3. Tumour Biol. 19(6): 480-7.
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