Human SIRP gamma/SIRPG HEK293 Overexpression Lysate: 产品信息
This Human SIRP gamma/SIRPG overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of SIRP gamma/SIRPG protein (Cat: 11828-H08H) from the overexpression lysate was verified.
A DNA sequence encoding the human SIRPG (NP_061026.2) extracellular domain (Met1-Ser364) was expressed with a polyhistidine tag at the C-terminus.
The recombinant human SIRPG consists of 347 amino acids and predictes a molecular mass of 38.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of human SIRPG is approximately 45-48 kDa due to glycosylation.
Human SIRP gamma/SIRPG HEK293 Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human SIRP gamma/SIRPG HEK293 Overexpression Lysate: 别称
Human bA77C3.1 Overexpression Lysate; Human CD172g Overexpression Lysate; Human SIRP-B2 Overexpression Lysate; Human SIRPB2 Overexpression Lysate; Human SIRPgamma Overexpression Lysate
SIRP gamma/SIRPG 背景信息
Signal-regulatory protein gamma (SIRPG/SIRP gamma) also known as CD172 antigen-like family member B, CD172g, and CD172g antigen, is a member of the signal-regulatory protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. SIRPG/SIRP gamma/CD172g is a probable immunoglobulin-like cell surface receptor. On binding with CD47, SIRPG can mediate cell-cell adhesion. SIRPG/SIRP gamma is engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation. SIRPG/SIRP gamma/CD172g is detected in liver, and at very low levels in brain, heart, lung, pancreas, kidney, placenta and skeletal muscle. Expressed on CD4+ T-cells, CD8+ T-cells, CD56-bright natural killer (NK) cells, CD20+ cells, and all activated NK cells. This cytokine is mainly present in the paracortical T-cell area of lymph nodes, with only sparse positive cells in the mantle and in the germinal center of B-cell follicles. In the thymus, SIRPG is primarily expressed in the medulla on mature T-lymphocytes that have undergone thymic selection.
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