This Human SLITRK4 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of SLITRK4 protein (Cat: 13147-H08H) from the overexpression lysate was verified.
A DNA sequence encoding the human SLITRK4 (Q8IW52) (Met 1-Pro 616) was fused with a polyhistidine tag at the C-terminus.
The secreted recombinant human SLITRK4 comprises 609 amino acids and has a predicted molecular mass of 68.6 kDa. The apparent molecular mass of rhSLITRK4 is approximately 85 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Human SLITRK4 HEK293 Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human SLITRK4 HEK293 Overexpression Lysate: 别称
Human DKFZp547M2010 Overexpression Lysate
SLIT and NTRK-like family proteins 4 is a member of the SLIT and NTRK-like family. This protein is characterized as an integral membrane protein that has two leucine-rich repeat (LRR) domains and a carboxy-terminal domain that is partially similar to SLIT protein and TRK Neurotrophin There are six members in this family: SLITRK1, SLITRK2, SLITRK3, SLITRK4, SLITRK5, and SLITRK6. Their expression was detected mainly in the brain, but the expression profile of each SLITRK was unique. Each of these members is located in nervous system except for SLITRK6. SLITRK expression was also investigated in various types of brain tumor tissue.
SLIT and NTRK like family member 4
Beaubien F, et al. (2009) Differential expression of slitrk family members in the mouse nervous system. Developmental Dynamics. 238 (12) : 3285-96.
Aruga J, et al. (2003) Human SLITRK family genes: genomic organization and expression profiling in normal brain and brain tumor tissue. Gene. 315 (2): 87-94.
Milde T, et al. (2007) A novel family of slitrk genes is expressed on hematopoietic stem cells and leukemias. Leukemia. 21: 824-7.