Human SorCS1 HEK293 Overexpression Lysate


Human SorCS1 HEK293 Overexpression Lysate: 产品信息

This Human SorCS1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of SorCS1 protein (Cat: 11948-H08H) from the overexpression lysate was verified.
HEK293 Cells
A DNA sequence encoding the human SORCS1 (Q8WY21-1) extracellular domain (Ser 111-Ser 1099) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus
The recombinant human SORCS1 consists of 1000 amino acids and predictes a molecular mass of 113 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhSORCS1 is approximately 130 kDa due to glycosylation

Human SorCS1 HEK293 Overexpression Lysate: 使用指南

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human SorCS1 HEK293 Overexpression Lysate: 别称

Human hSorCS Overexpression Lysate; Human RP11-446H13.1 Overexpression Lysate

SorCS1 背景信息

VPS1 domain-containing receptor SorCS1, also known as SORCS1 and SORCS, is a single-pass type I membrane protein that belongs to the SORCS family and SORCS1 subfamily. SORCS1 contains five BNR repeats and one PKD domain. SorCS1 is a member of the Vps1p-domain receptor family comprised of Sortilin, SorCS1, SorCS2, SorCS3, and SorLA. The common characteristic of these receptors is an N-terminal Vps1p domain, which either represents the only module of the luminal/extracellular moiety or is combined with additional domains. Family members play roles in protein transport and signal transduction. The individual receptors bind and internalize a variety of ligands, such as neuropeptides and trophic factors, and Sortilin and SorLA mediate trans-Golgi network-to-endosome sorting. Their prominent neuronal expression, several of the identified ligands, and results support the notion that members of this receptor family have important functions in neurogenesis, plasticity-related processes, and functional maintenance of the nervous system. Sortilin and SorLA mediate intracellular protein trafficking and sorting. SorCS1 binds platelet-derived growth factor-BB (PDGF-BB) and is expressed in isoforms differing only in their cytoplasmic domains. SorCS1 binds platelet-derived growth factor, a growth factor crucial for pericyte recruitment to the microvasculature, and may thus have a role in expanding or maintaining the islet vasculature.
sortilin-related VPS10 domain containing receptor 1
  • Hermey,G. et al., 2001, Neurosci Lett. 313 (1-2): 83-7.
  • Clee,S.M. et al., 2006, Nat Genet. 38 (6): 688-93.
  • Goodarzi,M.O. et al., 2007, Diabetes. 56 (7):1922-9.
  • Hermey,G. 2009, Cell Mol Life Sci. 66 (16):2677-89.
  • Reitz,C. et al., 2011, Ann Neurol. 69 (1):47-64.
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后 U.S.A.