Human Syndecan-2 HEK293 Overexpression Lysate


Human Syndecan-2 HEK293 Overexpression Lysate: 产品信息

This Human Syndecan-2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Syndecan-2 protein (Cat: 10355-H08H) from the overexpression lysate was verified.
HEK293 Cells
A DNA sequence encoding the human SDC2 (NP_002989.2) (Met1-Glu144) was expressed with a polyhistidine tag at the C-terminus.
The recombinant human SDC2 consists 137 amino acids and predicts a molecular mass of 15.4 kDa.

Human Syndecan-2 HEK293 Overexpression Lysate: 使用指南

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human Syndecan-2 HEK293 Overexpression Lysate: 别称

Human CD362 Overexpression Lysate; Human HSPG Overexpression Lysate; Human HSPG1 Overexpression Lysate; Human SYND2 Overexpression Lysate

Syndecan-2 背景信息

The key players in the miR-20a-5p/SDC2 axis may be a potential diagnostic biomarker and therapeutic target for OS patients. SDC2 methylation as a blood-based DNA test for early detection of colorectal cancer (CRC).
syndecan 2
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