Human Syntaxin 8 HEK293 Overexpression Lysate: 产品信息
This Human Syntaxin 8 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Syntaxin 8 protein (Cat: 14243-H07H) from the overexpression lysate was verified.
A DNA sequence encoding the human STX8 (Q9UNK0) (Met1-Gly215) was expressed with an N-terminal polyhistidine tag.
The recombinant human STX8 comprises 235 amino acids and has a predicted molecular mass of 27 kDa. The apparent molecular mass of the protein is approximately 34 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Human Syntaxin 8 HEK293 Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human Syntaxin 8 HEK293 Overexpression Lysate: 别称
Human CARB Overexpression Lysate
Syntaxin 8 背景信息
STX8, also known as syntaxin 8, directly interacts with HECTd3. STX8 forms the SNARE complex with syntaxin 7, vti1b and endobrevin. STX8 belongs to the syntaxin family. Members of this family are key molecules implicated in diverse vesicle docking and membrane fusion events. STX8 physically interacts with cystic fibrosis transmembrane conductance regulator (CFTR): recombinant syntaxin 8 binds CFTR in vitro and both proteins co-immunoprecipitate in HT29 cells. Syntaxin 8 regulates CFTR-mediated currents in chinese hamster ovary (CHO) cells stably expressing CFTR and syntaxin 8. STX8 contributes to the regulation of CFTR trafficking and chloride channel activity by the SNARE machinery.
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