This Human TMED4 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of TMED4 protein (Cat: 13729-H08H) from the overexpression lysate was verified.
A DNA sequence encoding the human TMED4 (Q7Z7H5-1) (Met1-Arg194) was expressed with a polyhistidine tag at the C-terminus.
The recombinant human TMED4 consists of 176 amino acids and predicts a molecular mass of 20.7 KDa. It migrates as an approximately 23 KDa band in SDS-PAGE under reducing conditions.
Human TMED4 HEK293 Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human TMED4 HEK293 Overexpression Lysate: 别称
Human ERS25 Overexpression Lysate; Human GMP25iso Overexpression Lysate; Human HNLF Overexpression Lysate; Human p24alpha3 Overexpression Lysate
TMED4, also known as ERS25, belongs to the EMP24/GP25L family. TMED4 may play a role in the regulation of heat-shock response and apoptosis. It is involved in vesicular protein trafficking, mainly in the early secretory pathway. TMED4 may also play a role in the biosynthesis of secreted cargo including processing. It functions in the regulation of heat-shock response and apoptosis. TMED4 also is involved in endoplasmic reticulum stress response.
transmembrane emp24 protein transport domain containing 4
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Matsuda A. et al., 2003, Oncogene. 22 (21): 3307-18.