Human TRF1 Baculovirus-Insect cells Overexpression Lysate


Human TRF1 Baculovirus-Insect cells Overexpression Lysate: 产品信息

This Human TRF1 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of TRF1 protein (Cat: 13044-H07B) from the overexpression lysate was verified.
Baculovirus-Insect cells
A DNA sequence encoding the human TERF1 isoform 2 (NP_003209.2) (Met 1-Asp 419) was expressed, with a polyhistidine tag at the N-terminus.
The recombinant human TERF1 consists of 438 amino acids and predicts a molecular mass of 50.5 kDa. It migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions.

Human TRF1 Baculovirus-Insect cells Overexpression Lysate: 使用指南

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human TRF1 Baculovirus-Insect cells Overexpression Lysate: 别称

Human FLJ41416 Overexpression Lysate; Human hTRF1-AS Overexpression Lysate; Human PIN2 Overexpression Lysate; Human t-TRF1 Overexpression Lysate; Human TRBF1 Overexpression Lysate; Human TRF Overexpression Lysate; Human TRF1 Overexpression Lysate

TRF1 背景信息

Telomeric repeat binding factor 1 (TRF1), also known as TERF1, the shelterin complex, which modulates the telomere structures. TRF1 protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Pin2/TRF1 was originally identified as a protein bound to telomeric DNA (TRF1) and as a protein involved in mitotic regulation (Pin2). Pin2/TRF1 negatively regulates telomere length and importantly, its function is tightly regulated during the cell cycle, acting as an important regulator of mitosis. TRF1 can be bound and modulated by two nucleolar GTP-binding proteins, nucleostemin (NS) and guanine nucleotide binding protein-like 3-like (GNL3L), which exhibit apparently opposite effects on the protein degradation of TRF1. TRF1/TERF1 may has associated with cancer. TRF1 may play a significant role in cell differentiation in non-small cell lung cancer (NSCLC). The expression level of TRF1 protein is significantly reduced in kidney cancer and the level is negatively correlated with malignant degree of the cancer. TRF1 expression in malignant gliomas cells, may play a role in the malignant progression of astroglial brain tumors.
telomeric repeat binding factor (NIMA-interacting) 1
  • Tsai RY. (2009) Nucleolar modulation of TRF1: a dynamic way to regulate telomere and cell cycle by nucleostemin and GNL3L. Cell Cycle. 8(18):2912-6.
  • Chen YC, et al. (2009) Phosphorylation of telomeric repeat binding factor 1 (TRF1) by Akt causes telomere shortening. Cancer Invest. 27(1): 24-8.
  • Hu J, et al. (2006) Expression of telomeric repeat binding factor 1 in non-small cell lung cancer. J Surg Oncol. 93(1): 62-7.
  • La Torre D, et al. (2005) Expression of telomeric repeat binding factor-1 in astroglial brain tumors. Neurosurgery. 56(4): 802-10.
  • Shi JM, et al. (2004) Expression of telomere repeat binding factor 1 (TRF1) protein in kidney cancer. Zhejiang Da Xue Xue Bao Yi Xue Ban. 33(6): 496-9, 508.
  • Zhou XZ, et al. (2003) Role of Pin2/TRF1 in telomere maintenance and cell cycle control. J Cell Biochem. 89(1): 19-37.
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