This Human TSPAN31 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of TSPAN31 protein (Cat: 14231-H04H) from the overexpression lysate was verified.
A DNA sequence encoding the human TSPAN31 (Q12999) (Cys94-Lys173) was expressed with the Fc region of mouse IgG1 at the N-terminus.
The recombinant human TSPAN31/mFc comprises 316 amino acids and has a predicted molecular mass of 35.9 kDa. The apparent molecular mass of the monomer is approximately 40-46 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Human TSPAN31 HEK293 Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human TSPAN31 HEK293 Overexpression Lysate: 别称
Human SAS Overexpression Lysate
TSPAN31 is a member of the transmembrane 4 superfamily. Most members of this family are cell-surface proteins that are characterized by the presence of four hydrophobic domains. They mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. TSPAN31 is thought to be involved in growth-related cellular processes. This gene is associated with tumorigenesis and osteosarcoma.
Wright MD. et al., 1995, Immunol Today. 15 (12): 588-94.
Meltzer PS. et al., 1992, Cell Growth Differ. 2 (10): 495-501.
Jankowski SA. et al., 1995, Genomics. 25 (2): 501-6.