Human ULBP4 HEK293 Overexpression Lysate

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Human ULBP4 HEK293 Overexpression Lysate: 产品信息

产品描述
This Human ULBP4 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of ULBP4 protein (Cat: 16073-H02H) from the overexpression lysate was verified.
表达宿主
HEK293 Cells
种属
Human
蛋白构建信息
A DNA sequence encoding the human RAET1E (NP_631904.1) (Met1-Asp225) was expressed with the Fc region of human IgG1 at the C-terminus.
分子量
The recombinant human RAET1E consists 433 amino acids and predicts a molecular mass of 49.1 kDa.

Human ULBP4 HEK293 Overexpression Lysate: 使用指南

制备方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解缓冲液
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
使用建议
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
缓冲液
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
应用
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human ULBP4 HEK293 Overexpression Lysate: 别称

Human bA350J20.7 Overexpression Lysate; Human LETAL Overexpression Lysate; Human N2DL-4 Overexpression Lysate; Human NKG2DL4 Overexpression Lysate; Human RAET1E2 Overexpression Lysate; Human RL-4 Overexpression Lysate; Human ULBP4 Overexpression Lysate

ULBP4 背景信息

The expressions of both RAET1E2 transcripts and protein can be found in different tumor cells and tissues.
全称
retinoic acid early transcript 1E
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