Human ULBP4 HEK293 Overexpression Lysate


Human ULBP4 HEK293 Overexpression Lysate: 产品信息

This Human ULBP4 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of ULBP4 protein (Cat: 16073-H02H) from the overexpression lysate was verified.
HEK293 Cells
A DNA sequence encoding the human RAET1E (NP_631904.1) (Met1-Asp225) was expressed with the Fc region of human IgG1 at the C-terminus.
The recombinant human RAET1E consists 433 amino acids and predicts a molecular mass of 49.1 kDa.

Human ULBP4 HEK293 Overexpression Lysate: 使用指南

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human ULBP4 HEK293 Overexpression Lysate: 别称

Human bA350J20.7 Overexpression Lysate; Human LETAL Overexpression Lysate; Human N2DL-4 Overexpression Lysate; Human NKG2DL4 Overexpression Lysate; Human RAET1E2 Overexpression Lysate; Human RL-4 Overexpression Lysate; Human ULBP4 Overexpression Lysate

ULBP4 背景信息

The expressions of both RAET1E2 transcripts and protein can be found in different tumor cells and tissues.
retinoic acid early transcript 1E
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后 U.S.A.