This Human VSTM1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of VSTM1 protein (Cat: 13171-H02H) from the overexpression lysate was verified.
A DNA sequence encoding the human VSTM1 (Q6UX27-16) extracellular domain (Met 1-Thr 135) was fused with the Fc region of human IgG1 at the C-terminus.
The recombinant human VSTM1/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 360 amino acids and has a predicted molecular mass of 40.6 kDa. The apparent molecular mass of the reduced monomer is approximately 50-60 kDa in SDS-PAGE under reducing conditions.
Human VSTM1 HEK293 Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human VSTM1 HEK293 Overexpression Lysate: 别称
Human MGC119160 Overexpression Lysate; Human MGC119161 Overexpression Lysate; Human SIRL-1 Overexpression Lysate; Human SIRL1 Overexpression Lysate; Human UNQ3033 Overexpression Lysate; Human VSTM1 Overexpression Lysate
V-set and transmembrane domain containing 1 (VSTM1) is a protein containing the V-set domains. V-set domains are immunoglobulin-like domains resembling the antibody variable region. V-set domains are found in many kinds of protein families, including immunoglobulin light and heavy chains, several T-cells such as CD2, CD4, CD80, and CD86, myelin membrane adhesion molecules, junction adhesion molecules (JAM), tyrosine-protein kinase receptors, and the programmed cell death protein1.
V-set and transmembrane domain containing 1
Satow Y, et al. (1986) Phosphocholine binding immunoglobulin Fab McPC603. An X-ray diffraction study at 2.7 A. J Mol Biol. 190 (4): 593-604.
Rees DC, et al. (2009) ABC transporters: the power to change. Cell Biol. 10 (3): 218-227.