This Mouse CD200R4 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CD200R4 protein (Cat: 50639-M08H) from the overexpression lysate was verified.
A DNA sequence encoding the extracellular domain of mouse CD200R4 (NP_997127.1) (Met 1-Thr 241) was expressed, fused with a polyhistidine tag at the C-terminus.
The secreted recombinant mouse CD200R4 comprises 227 amino acids and has a predicted molecular mass of 25 kDa. As a result of glycosylation, the apparent molecular mass of rm CD200R4 is approximately 50-60 kDa in SDS-PAGE under reducing conditions.
Mouse CD200R4 HEK293 Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Cell surface glycoprotein CD2 receptor 4, also known as Cell surface glycoprotein OX2 receptor 4, CD2 cell surface glycoprotein receptor-like 4, CD2RLa, and CD2R4, is a single-pass type I membrane protein which belongs to theCD2R family. CD2 (OX2) is a cell surface glycoprotein that interacts with a structurally related receptor (CD2R) expressed mainly on myeloid cells and is involved in regulation of macrophage and mast cell function. In mouse there are up to five genes related to CD2R with conflicting data as to whether they bind CD2. CD2R4 contains oneIg-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. CD2R4 is highly expressed in monocytes, NK cells and a subset of NKT cells. It is weakly expressed in granulocytes and B cells (at protein level). CD2R4 is also expressed in brain, lung, testis, thymus, intestine and uterus. and in bone marrow derived-macrophage and dendritic cells and mast cells. CD2R4 is involved in the recruitment or surface expression of the TYROBP receptor.
CD200 receptor 4
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