Mouse CD32B/Fcgr2b HEK293 Overexpression Lysate


Mouse CD32B/Fcgr2b HEK293 Overexpression Lysate: 产品信息

This Mouse CD32B/Fcgr2b overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CD32B/Fcgr2b protein (Cat: 50030-M27H) from the overexpression lysate was verified.
HEK293 Cells
A DNA sequence encoding the extracellular domain (Met 1-Arg 217) of mouse FGGR2B (NP_001070657.1) as fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus.
The secreted recombinant mouse FGGR2B consists of 212 amino acids and has a predicted molecular mass of 23.9 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FGGR2B is approximately 38-43 kDa due to glycosylation.

Mouse CD32B/Fcgr2b HEK293 Overexpression Lysate: 使用指南

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Mouse CD32B/Fcgr2b HEK293 Overexpression Lysate: 别称

Mouse AI528646 Overexpression Lysate; Mouse CD32 Overexpression Lysate; Mouse F630109E10Rik Overexpression Lysate; Mouse Fcgr2 Overexpression Lysate; Mouse Fcgr2a Overexpression Lysate; Mouse FcgRII Overexpression Lysate; Mouse Fcr-2 Overexpression Lysate; Mouse Fcr-3 Overexpression Lysate; Mouse fcRII Overexpression Lysate; Mouse Fc[g]RII Overexpression Lysate; Mouse Ly-17 Overexpression Lysate; Mouse Ly-m20 Overexpression Lysate; Mouse LyM-1 Overexpression Lysate

CD32B/Fcgr2b 背景信息

Fc fragment of IgG, low affinity IIb, receptor (CD32)
Related Pathways
  • B Cell Receptor Signaling Pathway
    B Cell Receptor Signaling Pathway
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