This Mouse CD52 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CD52 protein (Cat: 50721-M02H) from the overexpression lysate was verified.
A DNA sequence encoding the mouse Cd52 (NP_038734.1) (Met1-Lys46) was expressed with the Fc region of human IgG1 at the C-terminus.
The recombinant mouse Cd52 consists 261 amino acids and predicts a molecular mass of 29 kDa.
Mouse CD52 HEK293 Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
CD52 / CDW52 is a small glycosylphosphatidylinositol (GPI) anchored glycoprotein. It has a mature peptide comprising only 12 amino acids and is abundantly expressed on human lymphocytes. From the clinical point of view this protein is an important target for therapeutic interventions aimed at leukocyte depletion in hematological malignancies and post-transplant immunosuppression. CD52 / CDW52 may play a role in carrying and orienting carbohydrate. It is an unusually good target for complement-mediated cell lysis.