Mouse CEACAM1 HEK293 Overexpression Lysate

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Mouse CEACAM1 HEK293 Overexpression Lysate: 产品信息

产品描述
This Mouse CEACAM1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CEACAM1 protein (Cat: 50571-M08H) from the overexpression lysate was verified.
表达宿主
HEK293 Cells
种属
Mouse
蛋白构建信息
A DNA sequence encoding the extracellular domain of mouse CEACAM1 (NP_001034274.1) (Met 1-Gly 428) was expressed, with a polyhistidine tag at the C-terminus.
分子量
The recombinant mouse CEACAM1 consists of 405 amino acids and has a predicted molecular mass of 45 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmCEACAM1 is approximately 70-100 kDa due to high glycosylation.

Mouse CEACAM1 HEK293 Overexpression Lysate: 使用指南

制备方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解缓冲液
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
使用建议
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
缓冲液
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
应用
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Mouse CEACAM1 HEK293 Overexpression Lysate: 别称

Mouse bb-1 Overexpression Lysate; Mouse Bgp Overexpression Lysate; Mouse Bgp1 Overexpression Lysate; Mouse C-CAM Overexpression Lysate; Mouse Cc1 Overexpression Lysate; Mouse CD66a Overexpression Lysate; Mouse Cea-1 Overexpression Lysate; Mouse Cea-7 Overexpression Lysate; Mouse Cea1 Overexpression Lysate; Mouse Cea7 Overexpression Lysate; Mouse Hv-2 Overexpression Lysate; Mouse Hv2 Overexpression Lysate; Mouse mCEA1 Overexpression Lysate; Mouse Mhv-1 Overexpression Lysate; Mouse MHVR Overexpression Lysate; Mouse MHVR1 Overexpression Lysate; Mouse mmCGM1 Overexpression Lysate; Mouse mmCGM1a Overexpression Lysate; Mouse mmCGM2 Overexpression Lysate

CEACAM1 背景信息

The carcinoembryonic-antigen-related cell-adhesion molecule (CEACAM) family of proteins has been implicated in various intercellular-adhesion and intracellular-signalling-mediated effects that govern the growth and differentiation of normal and cancerous cells. CEACAM1, also known as biliary glycoprotein I (BGP I) and CD66a, is a member of the carcinoembryonic antigen (CEA) gene family which belongs to the immunoglobulin superfamily. The highly glycosylated CEACAM1 contains one N-terminal V-type Ig-like domain and three C2-type Ig-like domains within its ECD, and one ITIM motif and a calmodulin binding site in the cytoplasmic region. CEACAM1 is a surface glycoprotein expressed on various blood cells, epithelial cells, and vascular cells. It was described as an adhesion molecule mediating cell adhesion via both homophilic and heterophilic manners, and was detected on leukocytes, epithelia, and endothelia. Studies have revealed that CEACAM1 performs actions in multiple cellular processes including tissue differentiation, angiogenesis, apoptosis, metastasis, as well as the modulation of innate and adaptive immune responses.
全称
carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)
参考文献
  • Gray-Owen SD, et al. (2006) CEACAM1: contact-dependent control of immunity. Nat Rev Immunol. 6(6): 433-46.
  • Gu A, et al. (2009) Role of Ceacam1 in VEGF induced vasculogenesis of murine embryonic stem cell-derived embryoid bodies in 3D culture. Exp Cell Res. 315(10): 1668-82.
  • Wong C, et al. (2009) CEACAM1 negatively regulates platelet-collagen interactions and thrombus growth in vitro and in vivo. Blood. 113(8): 1818-28.
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