Mouse CHODL HEK293 Overexpression Lysate


Mouse CHODL HEK293 Overexpression Lysate: 产品信息

This Mouse CHODL overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CHODL protein (Cat: 50699-M08H) from the overexpression lysate was verified.
HEK293 Cells
A DNA sequence encoding the extracellular domain of mouse CHODL (EDK98299.1) (Met 1-Asn 216) was expressed, fused with a polyhistidine tag at the C-terminus.
The recombinant mouse CHODL comprises 206 amino acids and has a calculated molecular mass of 23.4 kDa. As a result of glycosylation, the apparent molecular mass of rnCHODL is approximately 34 kDa in SDS-PAGE under reducing conditions.

Mouse CHODL HEK293 Overexpression Lysate: 使用指南

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Mouse CHODL HEK293 Overexpression Lysate: 别称

Mouse 3110074E07Rik Overexpression Lysate; Mouse MT75 Overexpression Lysate; Mouse PRED12 Overexpression Lysate

CHODL 背景信息

  • Weng L, et al. (2003) A novel alternative spliced chondrolectin isoform lacking the transmembrane domain is expressed during T cell maturation. J Biol Chem. 278(21): 19164-70.
  • Masuda K, et al. (2011) Chondrolectin is a novel diagnostic biomarker and a therapeutic target for lung cancer. Clin Cancer Res. 17(24): 7712-22.
  • Weng L, et al. (2002) Molecular cloning and characterization of human chondrolectin, a novel type I transmembrane protein homologous to C-type lectins. Genomics. 80(1): 62-70.
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