Mouse FCGRT & B2M Heterodimer HEK293 Overexpression Lysate


Mouse FCGRT & B2M Heterodimer HEK293 Overexpression Lysate: 产品信息

This Mouse FCGRT & B2M Heterodimer overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of FCGRT & B2M Heterodimer protein (Cat: CT029-M08H) from the overexpression lysate was verified.
HEK293 Cells
A DNA sequence encoding the extracellular domain (Met 1-Ser 297) of mouse FCGRT (Q61559) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the mouse B2M (P01887,105Ala/Asp) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified
The recombinant heterodimer of mouse FCGRT / B2M comprises 385 ( 285 + 100 ) amino acids and has a calculated molecular mass of 43.5 ( 31.8 + 11.7 ) kDa. The apparent molecular mass of FCGRT / B2M heterodimer is approximately 35 & 12 kDa respectively in SDS-PAGE under reducing conditions

Mouse FCGRT & B2M Heterodimer HEK293 Overexpression Lysate: 使用指南

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Mouse FCGRT & B2M Heterodimer HEK293 Overexpression Lysate: 别称

Mouse FcRn Overexpression Lysate

FCGRT & B2M Heterodimer 背景信息

Fc fragment of IgG, receptor, transporter, alpha
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