This Mouse FZD1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of FZD1 protein (Cat: 50092-M08H) from the overexpression lysate was verified.
A DNA sequence encoding the mouse FZD1 (NP_067432.2) extracellular domain (Met 1-His 248) was expressed with a polyhistidine tag at the C-terminus.
The secreted recombinant mouse FZD1 pro form consists of 191 amino acids and has a predicted molecular mass of 21 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FZD1 is approximately 35-40 kDa due to glycosylation.
Mouse FZD1 HEK293 Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Frizzled-1, also known as FZD1, belongs to theG-protein coupled receptor Fz/Smo family. FZD1 contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, 7 transmembrane domains, and a C-terminal PDZ domain-binding motif. FZD1 is expressed in adult heart, placenta, lung, kidney, pancreas, prostate, and ovary and in fetal lung and kidney. Frizzled is a family of G protein-coupled receptor proteins that serve as receptors in the Wnt signaling pathway and other signaling pathways. When activated, Frizzled leads to activation of Dishevelled in the cytosol. Frizzled proteins and the genes encoding them have been identified in an array of animals, from sponges to humans. Frizzled proteins play key roles in governing cell polarity, embryonic development, formation of neural synapses, cell proliferation, and many other processes in developing and adult organisms. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes.
frizzled class receptor 1
Non-Canonical Wnt Pathway
Actin Dynamics Signaling Pathway
AKT Signaling Pathway
P38 MAPK Signaling Pathway
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