Mouse H Cadherin HEK293 Overexpression Lysate: 产品信息
This Mouse H Cadherin overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of H Cadherin protein (Cat: 51163-M02H) from the overexpression lysate was verified.
A DNA sequence encoding the mouse Cdh13 (NP_062681.2) (Met1-Ala692) was expressed with the Fc region of human IgG1 at the C-terminus.
The recombinant mouse Cdh13 consists 911 amino acids and predicts a molecular mass of 100.6 kDa.
Mouse H Cadherin HEK293 Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
CDH13, also known as cadherin-13 and H Cadherin, is a member of the cadherin superfamily. CDH13 acts as a negative regulator of axon growth during neural differentiation. It also protects vascular endothelial cells from apoptosis due to oxidative stress, and is associated with resistance to atherosclerosis. CDH13 is localized to the surface of the cell membrane and is anchored by a GPI moiety, rather than by a transmembrane domain. CDH13 gene is hypermethylated in many types of cancer.
Canonical Wnt Pathway
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