Mouse Kallikrein 1 HEK293 Overexpression Lysate


Mouse Kallikrein 1 HEK293 Overexpression Lysate: 产品信息

This Mouse Kallikrein 1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Kallikrein 1 protein (Cat: 50915-M08H) from the overexpression lysate was verified.
HEK293 Cells
A DNA sequence encoding the mouse KLK1 (P15947) (Met1-Asp261) was expressed with a C-terminal polyhistidine tag.
The recombinant mouse KLK1 comprises 254 amino acids and has a predicted molecular mass of 28.3 kDa. The apparent molecular mass of the protein is approximately 36 kDa in SDS-PAGE under reducing conditions.

Mouse Kallikrein 1 HEK293 Overexpression Lysate: 使用指南

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Mouse Kallikrein 1 HEK293 Overexpression Lysate: 别称

Mouse 0610007D04Rik Overexpression Lysate; Mouse Kal Overexpression Lysate; Mouse Klk1b6 Overexpression Lysate; Mouse Klk6 Overexpression Lysate; Mouse mGk-6 Overexpression Lysate

Kallikrein 1 背景信息

KLK1 (Kallikrein 1) is a Protein Coding gene. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. This protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen. Human tissue kallikrein (KLK1) is a serine protease, a component of the KKS that has been demonstrated to exert pleiotropic beneficial effects in protection from tissue injury through its anti-inflammatory, antiapoptotic, antifibrotic, and antioxidative actions. Polymorphism of the human tissue kallikrein 1 (KLK1) A1789G gene is associated with susceptibility to hypertension. Diseases associated with KLK1 include Kallikrein, Decreased Urinary Activity Of and Hereditary Angioedema.
kallikrein 1
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