Mouse MCP-1/CCL2 Baculovirus-Insect cells Overexpression Lysate

Price:
Size:
Number:

Mouse MCP-1/CCL2 Baculovirus-Insect cells Overexpression Lysate: 产品信息

产品描述
This Mouse MCP-1/CCL2 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of MCP-1/CCL2 protein (Cat: 50368-M08B) from the overexpression lysate was verified.
表达宿主
Baculovirus-Insect cells
种属
Mouse
蛋白构建信息
A DNA sequence encoding the mouse CCL2 (NP_035463.1) (Met1-Asn148) was expressed with a C-terminal polyhistidine tag.
分子量
The recombinant mouse CCL2 comprises 135 amino acids and has a predicted molecular mass of 15.2 kDa. The apparent molecular mass of the protein is approximately 27 kDa in SDS-PAGE under reducing conditions.

Mouse MCP-1/CCL2 Baculovirus-Insect cells Overexpression Lysate: 使用指南

制备方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解缓冲液
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
使用建议
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
缓冲液
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
应用
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Mouse MCP-1/CCL2 Baculovirus-Insect cells Overexpression Lysate: 别称

Mouse AI323594 Overexpression Lysate; Mouse HC11 Overexpression Lysate; Mouse JE Overexpression Lysate; Mouse MCAF Overexpression Lysate; Mouse MCP-1 Overexpression Lysate; Mouse MCP1 Overexpression Lysate; Mouse Scya2 Overexpression Lysate; Mouse Sigje Overexpression Lysate; Mouse SMC-CF Overexpression Lysate

MCP-1/CCL2 背景信息

Monocyte chemoattractant protein 1 (MCP-1), also called CCL2, belongs to a group of CC chemokines located in chromosome 17q11.2. MCP-1 protein interacts with chemokine C-C motif receptor 2 (CCR2) to activate and recruit monocytes, macrophages, CD4+ T cells and immature dendritic cells to the site of infection. The presence of MCP-1 protein in an adequate concentration is important for granuloma formation and M. tuberculosis clearance.
全称
chemokine (C-C motif) ligand 2
研究领域
Related Pathways
  • IL17 signaling pathway
    IL17 signaling pathway
参考文献
  • Vásquez-Loarte T, Trubnykova M, Guio H. Genetic association meta-analysis: a new classification to assess ethnicity using the association of MCP-1 -2518 polymorphism and tuberculosis susceptibility as a model. BMC Genetics. 2015;16:128.
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后 U.S.A.