Mouse NK1.1/KLRB1F HEK293 Overexpression Lysate


Mouse NK1.1/KLRB1F HEK293 Overexpression Lysate: 产品信息

This Mouse NK1.1/KLRB1F overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of NK1.1/KLRB1F protein (Cat: 50815-M01H) from the overexpression lysate was verified.
HEK293 Cells
A DNA sequence encoding the extracellular domain of mouse KLRB1F (Q8VD98) (Gln 67-Val 217) was fused with the Fc region of human IgG1 at the N-terminus.
The recombinant mouse KLRB1F/Fc is a disulfide-linked homodimer. The reduced monomer comprises 411 amino acids and has a calculated molecular mass of 46 kDa. As a result of glycosylation, the apparent molecular mass of the monomer is approximately 52 kDa in SDS-PAGE under reducing conditions.

Mouse NK1.1/KLRB1F HEK293 Overexpression Lysate: 使用指南

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Mouse NK1.1/KLRB1F HEK293 Overexpression Lysate: 别称

Mouse A630024B12Rik Overexpression Lysate; Mouse MGC130065 Overexpression Lysate; Mouse MGC130066 Overexpression Lysate; Mouse Nkr-p1f Overexpression Lysate; Mouse Nkrp1-f Overexpression Lysate; Mouse Nkrp1f Overexpression Lysate

NK1.1/KLRB1F 背景信息

killer cell lectin-like receptor subfamily B member 1F
  • Kveberg L, et al. (2011) Phylogenetic and functional conservation of the NKR-P1F and NKR-P1G receptors in rat and mouse. Immunogenetics. 63(7): 429-36.
  • Aust JG, et al. (2009) The expression and function of the NKRP1 receptor family in C57BL/6 mice. J Immunol. 183(1): 106-16.
  • Tian W, et al. (2005) C-type lectin OCILRP2/Clr-g and its ligand NKRP1f costimulate T cell proliferation and IL-2 production. Cell Immunol. 234(1): 39-53.
  • Plougastel B, et al. (2001) Analysis of a 1-Mb BAC contig overlapping the mouse Nkrp1 cluster of genes: cloning of three new Nkrp1 members, Nkrp1d, Nkrp1e, and Nkrp1f. Immunogenetics. 53(7): 592-8.
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