Mouse PILRA HEK293 Overexpression Lysate


Mouse PILRA HEK293 Overexpression Lysate: 产品信息

This Mouse PILRA overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of PILRA protein (Cat: 50234-M08H) from the overexpression lysate was verified.
HEK293 Cells
A DNA sequence encoding the mouse PILRA (NP_705730.1) extracellular domain (Met 1-Val 197) was fused with a polyhistidine tag at the C-terminus.
The recombinant mouse PILRA consists of 177 amino acids and has a predicted molecular mass of 20.2 kDa. As a result of glycosylation, the apparent molecular mass of rm PILRA is approximately 35-45 kDa in SDS-PAGE under reducing conditions.

Mouse PILRA HEK293 Overexpression Lysate: 使用指南

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Mouse PILRA HEK293 Overexpression Lysate: 别称

Mouse AV021745 Overexpression Lysate; Mouse FDF03 Overexpression Lysate

PILRA 背景信息

paired immunoglobin-like type 2 receptor alpha
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