Mouse PLGF/PGF HEK293 Overexpression Lysate

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Mouse PLGF/PGF HEK293 Overexpression Lysate: 产品信息

产品描述
This Mouse PLGF/PGF overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of PLGF/PGF protein (Cat: 50125-MCCH) from the overexpression lysate was verified.
表达宿主
HEK293 Cells
种属
Mouse
蛋白构建信息
A DNA sequence encoding the mouse PGF (P49764) (Met1-Pro158) was fused with five amino acids (DDDDK) at the C-terminus was expressed and purified.
分子量
The recombinant mouse PGF consists of 146 amino acids and has a calculated molecular mass of 16.6 kDa. The recombinant protein migrates as an approximately 28 kDa band in SDS-PAGE under reducing conditions.

Mouse PLGF/PGF HEK293 Overexpression Lysate: 使用指南

制备方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解缓冲液
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
使用建议
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
缓冲液
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
应用
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Mouse PLGF/PGF HEK293 Overexpression Lysate: 别称

Mouse AI854365 Overexpression Lysate; Mouse PIGF Overexpression Lysate; Mouse Plgf Overexpression Lysate

PLGF/PGF 背景信息

全称
placental growth factor
参考文献
  • Nagy JA, et al. (2003) VEGF-A(164/165) and PLGF: roles in angiogenesis and arteriogenesis. Trends Cardiovasc Med. 13(5): 169-75.
  • Chaballe L, et al. (2011) Placental growth factor: a tissue modelling factor with therapeutic potentials in neurology? Acta Neurol Belg. 111(1): 10-7.
  • Odorisio T, et al. (2006) The placental growth factor in skin angiogenesis. J Dermatol Sci. 41(1): 11-9.
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