Mouse Serpina1d HEK293 Overexpression Lysate

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Mouse Serpina1d HEK293 Overexpression Lysate: 产品信息

产品描述
This Mouse Serpina1d overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Serpina1d protein (Cat: 51172-M02H) from the overexpression lysate was verified.
表达宿主
HEK293 Cells
种属
Mouse
蛋白构建信息
A DNA sequence encoding the mouse Serpina1a (XP_003945711.1) (Met1-Lys413) was expressed with the Fc region of human IgG1 at the C-terminus.
分子量
The recombinant mouse Serpina1a comprises 627 amino acids and has a predicted molecular mass of 70.2 kDa. The apparent molecular mass of the protein is approximately 67-77 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Mouse Serpina1d HEK293 Overexpression Lysate: 使用指南

制备方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解缓冲液
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
使用建议
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
缓冲液
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
应用
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Mouse Serpina1d HEK293 Overexpression Lysate: 别称

Mouse AI118301 Overexpression Lysate; Mouse Dom4 Overexpression Lysate; Mouse PI4 Overexpression Lysate; Mouse Serpin A1d Overexpression Lysate; Mouse Spi1-4 Overexpression Lysate

Serpina1d 背景信息

全称
serine (or cysteine) peptidase inhibitor, clade A, member 1D
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