Mouse ST6GALNAC2 HEK293 Overexpression Lysate

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Mouse ST6GALNAC2 HEK293 Overexpression Lysate: 产品信息

产品描述
This Mouse ST6GALNAC2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of ST6GALNAC2 protein (Cat: 50774-M08H) from the overexpression lysate was verified.
表达宿主
HEK293 Cells
种属
Mouse
蛋白构建信息
A DNA sequence encoding the extracellular domain of mouse ST6GALNAC2 (P70277) (Ser 28-Arg 373) was expressed, with a C-terminal polyhistidine tag.
分子量
The secreted recombinant mouse ST6GALNAC2 comprises 357 amino acids and has a calculated molecular mass of 40.7 kDa. The apparent molecular mass of rmST6GALNAC2 is approximately 44 kDa in SDS-PAGE under reducing conditions.

Mouse ST6GALNAC2 HEK293 Overexpression Lysate: 使用指南

制备方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解缓冲液
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
使用建议
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
缓冲液
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
应用
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Mouse ST6GALNAC2 HEK293 Overexpression Lysate: 别称

Mouse II Overexpression Lysate; Mouse Siat7 Overexpression Lysate; Mouse Siat7b Overexpression Lysate; Mouse ST6GalNAc Overexpression Lysate

ST6GALNAC2 背景信息

全称
ST6-N-acetylgalactosaminide alpha-2,6-sialyltransferase 2
参考文献
  • Li GS, et al. (2007) Variants of the ST6GALNAC2 promoter influence transcriptional activity and contribute to genetic susceptibility to IgA nephropathy. Hum Mutat. 28(10): 950-7.
  • Ding JX, et al. (2009) Activity of alpha2,6-sialyltransferase and its gene expression in peripheral B lymphocytes in patients with IgA nephropathy. Scand J Immunol. 69(2): 174-80.
  • Kiryluk K, et al. (2010) Genetic studies of IgA nephropathy: past, present, and future. Pediatr Nephrol. 25(11): 2257-68.
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