Mouse Transferrin Receptor/TFRC HEK293 Overexpression Lysate

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Mouse Transferrin Receptor/TFRC HEK293 Overexpression Lysate: 产品信息

产品描述
This Mouse Transferrin Receptor/TFRC overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Transferrin Receptor/TFRC protein (Cat: 50741-M07H) from the overexpression lysate was verified.
表达宿主
HEK293 Cells
种属
Mouse
蛋白构建信息
A DNA sequence encoding the extracellular domain of mouse TFRC (Q62351) (Cys 89-Phe 763) was fused with a polyhistidine tag at the N-terminus.
分子量
The recombinant mouse TFRC comprises 694 amino acids and predicts a molecular mass of 78.3 kDa. The apparent molecular mass of mouse TFRC is approximately 80 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Mouse Transferrin Receptor/TFRC HEK293 Overexpression Lysate: 使用指南

制备方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解缓冲液
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
使用建议
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
缓冲液
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
应用
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Mouse Transferrin Receptor/TFRC HEK293 Overexpression Lysate: 别称

Mouse 2610028K12Rik Overexpression Lysate; Mouse AI195355 Overexpression Lysate; Mouse AI426448 Overexpression Lysate; Mouse AU015758 Overexpression Lysate; Mouse CD71 Overexpression Lysate; Mouse E430033M20Rik Overexpression Lysate; Mouse Mtvr-1 Overexpression Lysate; Mouse Mtvr1 Overexpression Lysate; Mouse p90 Overexpression Lysate; Mouse TFR Overexpression Lysate; Mouse TFR1 Overexpression Lysate; Mouse TR Overexpression Lysate; Mouse Trfr Overexpression Lysate

Transferrin Receptor/TFRC 背景信息

Transferrin receptor protein 1, also known as transferrin receptor, Trfr, p9, CD71 and TFRC, is a single-pass type II membrane protein that belongs to the peptidase M28 family and M28B subfamily. TFRC / CD71 is a membrane-bound protein expressed in larger amounts in proliferating. The specific expression of TFRC can represent a diagnostic tool or a therapeutic target in solid tumours expressing this antigen. Transferrin receptor is necessary for development of erythrocytes and the nervous system. TFRC / CD71 is regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3'-UTR. Up-regulated upon mitogenic stimulation. TFRC / CD71 represents a marker of malignant transformation in the pancreas that could be applied as potential diagnostic and therapeutic target.
全称
transferrin receptor
参考文献
  • Douabin-Gicquel V., et al., 2001,Hum. Genet. 109:393-401.
  • Ryschich,E. et al., 2004,Eur J Cancer. 40 (9):1418-22.
  • Tosoni D., et al., 2005, Cell 123:875-888.
  • Wollscheid B., et al., 2009, Nat. Biotechnol. 27:378-386.
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