This Mouse TSLP overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of TSLP protein (Cat: 51005-M08H) from the overexpression lysate was verified.
A DNA sequence encoding the mouse TSLP (Q9JIE6) (Met 1-Glu 140) was expressed, with a C-terminal polyhistidine tag.
The secreted recombinant mouse TSLP comprises 132 amino acids and has a calculated molecular mass of 15.4 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 22-27 kDa in SDS-PAGE under reducing conditions.
Mouse TSLP HEK293 Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Mouse TSLP HEK293 Overexpression Lysate: 别称
Mouse TSLP Overexpression Lysate
Thymic stromal lymphopoietin (TSLP) is an interleukin 7 (IL-7)-like cytokine originally characterized by its ability to promote the activation of B cells and dendritic cells (DCs). Thymic stromal lymphopoietin (TSLP) is a cytokine expressed by epithelial cells, including keratinocytes, and is important in allergic inflammation. Subsequent studies have shown that TSLP promotes T helper type 2 (TH2) cell responses associated with immunity to some helminth parasites and the pathogenesis of many inflammatory diseases, including atopic dermatitis and asthma. TSLP can promote TH2 cytokine-associated inflammation by directly promoting the effector functions of CD4+ TH2 cells, basophils and other granulocyte populations while simultaneously limiting the expression of DC-derived proinflammatory cytokines and promoting regulatory T cell responses in peripheral tissues.
thymic stromal lymphopoietin
Comeau MR, et al. (2010) The influence of TSLP on the allergic response. Mucosal Immunol. 3 (2): 138-47.
Ziegler SF. (2010) The role of thymic stromal lymphopoietin (TSLP) in allergic disorders. Curr Opin Immunol. 22 (6): 795-9.
Liu YJ, et al. (2007) TSLP: An Epithelial Cell Cytokine that Regulates T Cell Differentiation by Conditioning Dendritic Cell Maturation. Annual Review of Immunology. 25: 193-219.
He R, et al. (2008) TSLP acts on infiltrating effector T cells to drive allergic skin inflammation.PNAS. 105 (33): 11875-80.