This Rat CLEC4B2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CLEC4B2 protein (Cat: 80265-R07H) from the overexpression lysate was verified.
A DNA sequence encoding the rat CLEC4B2 (Q67DU9) (Met44-Leu208) was expressed with a polyhistidine tag at the N-terminus.
The recombinant rat CLEC4B2 comprises 185 amino acids and has a predicted molecular mass of 21.8 kDa. The apparent molecular mass of the protein is approximately 31-36 kDa in SDS-PAGE under reducing conditions.
Rat CLEC4B2 HEK293 Overexpression Lysate: 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
稳定性 & 储存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Rat CLEC4B2 HEK293 Overexpression Lysate: 别称
Rat Aplra1 Overexpression Lysate; Rat CLEC4B2 Overexpression Lysate
Clec4b2, also known as mDCAR1, is a member of the DCIR/DCAR family. Expression of Clec4b2 was strongly tissue dependent. Clec4b2 expression on DCs was restricted to the CD8(+) DC subset in spleen and thymus and on subpopulations of CD11b(+) myeloid cells in bone marrow and spleen, whereas the molecule was not detectable on both cell types in lymph nodes and peripheral blood. Clec4b2 is a functional receptor on cells of the immune system and provides further insights into the regulation of immune responses by CLRs.
C-type lectin domain family 4, member b2
Katayama S. et al., 2005, Science. 309 (5740): 1564-6.
Kaden SA. et al., 2009, J Immunol. 183 (8): 5069-78.
Skarnes WC. et al., 2011, Nature. 474 (7351): 337-42.