West Nile Virus (WNV) (lineage 1, strain NY99) NS1 HEK293 Cell Lysate (WB positive control): 产品信息
This WNV WNV Nonstructural protein 1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of WNV Nonstructural protein 1 protein (Cat: 40346-V07H) from the overexpression lysate was verified.
A DNA sequence encoding the NS1 of West Nile Virus (WNV)(lineage 1, strain NY99) (YP_001527881.1) (Asp1-Ala352) was expressed with a polyhistidine tag at the N-terminus.
The recombinant the NS1 of West Nile Virus (WNV)(lineage 1, strain NY99) consists of 371 amino acids and predicts a molecular mass of 42.1 kDa.
West Nile Virus (WNV) (lineage 1, strain NY99) NS1 HEK293 Cell Lysate (WB positive control): 使用指南
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.