Mammalian Cell Expression Vector Construction Service Description

Your Most Reliable & Cost-Effective Gene & Vector Supporting Partner

     

Gene Cloning Gene Synthesis

 

Expression Vector Construction

 

Plasmid Production

 

qPCR Primer Design & Testing

Mammalian Cell Expression Vector Construction Service

Mammalian cells usually show low protein expression in comparison to microbial and yeast. However, a majority of protein expression studies are conducted in mammalian cells or in animals. Various methods have been attempted to improve protein expression in mammalian cells with various degrees of success. These methods include enhancement in protein expression promoter, insertion of enhance, insertion of intron, optimization of poly A sequence, etc.

Construction of mammalian cell expression vector is a mature technology, but it still takes significant amount of efforts and time to complete the task. It often can take a couple of weeks, if not months, for a first graduate student who is just starting to get hands wet in the laboratory. Most importantly, the commercial mammalian cell expression vector in most laboratories is not optimized and hence may not be suitable for high expression in mammalian cells or in animals.

Sino Biological is an expert in mammalian cell expression and large scale production. We have successfully produced over 4000 recombinant proteins in mammalian cells. We have extensively and systematically optimized mammalian cell expression vectors and have achieved excellent performance in various cell lines. We are offering our best in class mammalian cell expression vectors for non-commercial research purposes to support life science research. Our optimized pCMV3 vector has been validated in mammalian cells with over 500 genes with a success rate of over 98%.

In addition to our best performance mammalian cell expression vector backbone, we also offer a variety of detection and purification tag choices, such as FLAG, poly-his, GST, HA, Myc, etc. One can customize the vector design to suit specific purposes.

By outsourcing your protein expression vector construction work to us, you are taking advantages of our superior proprietary pCMV3 expression vector (free of charge for non-commercial use) and our professional staff's expertise and experience. It often saves significant amount of valuable research time and saves cost in the same time. Contact us now to learn more about our mammalian cell expression vector construction service.

How to Request for Mammalian Cell Expression Vector Construction Service?

If you are interested in using our mammalian cell expression vector construction service, please contact us by leaving an on-line message, filling out an on-line inquiry form, or sending us your detailed requests to CRO-service@sinobiological.com. A formal quote will be send back to you for your review, comment, and/or acceptance.

Biology CRO Service
Biology CRO Service Introduction
Antibody Production & Development CRO Service
Protein Production CRO Service
Transient Transfection Service: Mammalian Cell Culture
Molecular Biology CRO Service
Gene Expression Vector Construction CRO Service+
- Gene Expression Vector Construction Service Description
- Mammalian Cell Expression Vector Construction Service
Mammalian Cell Expression Vector Construction Service Description
Mammalian Expression Vector Construction Service: pCMV3-N-FLAG
Mammalian Expression Vector Construction Service: pCMV3-N-His
Mammalian Expression Vector Construction Service: pCMV3-N-HA
Mammalian Expression Vector Construction Service: pCMV3-N-Myc
Mammalian Expression Vector Construction Service: pCMV3-N-GFPSpark
Mammalian Expression Vector Construction Service: pCMV3-N-OFPSpark
Mammalian Expression Vector Construction Service: pCMV3-C-FLAG
Mammalian Expression Vector Construction Service: pCMV3-C-His
Mammalian Expression Vector Construction Service: pCMV3-C-HA
Mammalian Expression Vector Construction Service: pCMV3-C-Myc
Mammalian Expression Vector Construction Service: pCMV3-C-GFPSpark
Mammalian Expression Vector Construction Service: pCMV3-C-OFP
Mammalian Expression Vector Construction Service: untagged
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- E. Coli. Expression Vector Construction Service
- Yeast Expression Vector Construction Service