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A very high percentage of Mouse monoclonal antibodies used for research are mouse IgG1 antibodies. Mouse antibody purification from either hybridoma cell culture or transient transfected 293 or CHO cell cultures may not be as straight forward as it looks. Often it can be troublesome due to low binding affinity of some mouse antibodies to protein A and protein G resin, especially for IgG1 and IgG3b antibodies. Precautions need to be taken to avoid antibody flow-through in protein A and protein G affinity purification. Optimization of the loading buffer can increase the binding efficiency of mouse antibodies to protein A and protein G resins, and hence can increase the yield of mouse antibody recovery from cell culture supernatant.
Sino Biological has produced and purified thousands of mouse monoclonal antibodies either in mouse hybridoma cell cultures or transient transfection in HEK 293 cells. Our hybridoma cell culture and transient transfection capacity is virtually unlimited for any scale of mouse IgG antibody production and purification. Our professional mouse IgG1 antibody production team is ready to serve you in meeting your antibody production needs.
How to Submit a Request for Antibody Production and Purification Service?
If you are interested in using our antibody purification services to support your antibody discovery and development program, please contact us by leaving an on-line message or send us your detailed request to CROfirstname.lastname@example.org. Based on the quantity and quality requirement of your request, a formal quote will be send back to you for your review, comment, and/or acceptance.