The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
储存 & 运输
Lyophilized qPCR primer mix is shipped at ambiente temperatura
The lyophilized product is stable for one year from date of receipt when stored at -20℃.
The suspended product is stable for six months from date of receipt when stored at -20℃.
***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***
在特定基因的不同变体保守区内设计引物，该对引物至少有一条引物跨越内含子或产物跨越内含子，有效避免基因组 DNA 的扩增。
用质粒标准品对 qPCR 引物的灵敏度、扩增效率和特异性进行筛选，用阳性组织或细胞验证确认。
统一的 PCR 条件，操作方便，节约时间与成本
~100% 的扩增效率，保证 RNA 定量的准确性
Integrin beta 3 qPCR Primer Pairs, Mouse: 别称
CD61 qPCR Primer Pairs, Mouse; GP3A qPCR Primer Pairs, Mouse; INGRB3 qPCR Primer Pairs, Mouse
Integrin beta 3 背景信息
Integrins containing the beta(3) subunit are key players in tumor growth and metastasis.ITGB3 plays an important role in ROS-induced migration and invasion in Colorectal cancer (CRC).ITGB3 is translationally activated in hypoxia and regulates malignant features, including epithelial-mesenchymal transition and cell migration, through the TGF-beta pathway, revealing a novel angle for the treatment of therapy-resistant hypoxic tumours.Bioinformatic algorithms and luciferase reporter assays revealed that integrin beta3 (ITGB3) is a direct target of miR-3a-5p.