Mouse Mab Production Description

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Mouse Monoclonal Antibody Production Service Description

In addition to high-quality mouse antibody development service, Sino Biological also offers professional cost-effective mouse antibody production service. For mouse antibody production request from a hybridoma cell line, the first choice is to expand the cell line for large scale cell culture and antibody production. In many cases, it is a simple and fast process, especially for small scale production. However, quite often, many hybridoma cell lines are troublesome with stability, contamination, mixed clones, etc. An unstable hybridoma cell line will make it really difficult or even impossible to produce a large quantity of antibody, because in the process of expanding cells through multiple passages, the cell line's antibody productivity can quickly decrease. Sino Biological is highly experienced in producing mouse antibodies from virtually any hybridoma cell lines.

Our starting point can be a hybridoma cell line deposited in our cell bank if the antibody was developed using our service, or a cryo-vial of hybridoma cells shipped to us, or mouse antibody VL and VH gene sequence. For mouse antibody production from mouse hybridoma cell culture, Sino Biological has extensive experience in fed-batch culture of hybridoma cells from different customers as well as experience in adapting cells originally cultured and banked in serum-containing medium to low serum or serum-free conditions. Our expertise also includes know-how and experience in purifying mouse IgG1, IgG2a, IgG2b, IgG3, IgA, and IgM antibodies.

For mouse antibody production by transient transfection, Sino Biological is highly experienced in handling such situations. We have developed expertise in house to clone the antibody genes from the hybridoma cell line and use our recombinant technology to produce the antibody by transient transfection. The strategy also works perfectly well with hybridoma cell lines that have low level of microbial or mycoplasma contamination, which prevent one from expanding the cells for large scale production. Our large scale transient transfection in HEK293 cells or CHO cells or in stable CHO cells can meet mouse antibody production needs at any scale.

We have capacity to grow mouse hybridoma cells at high-throughput at small scale as well as in large scale stirred-tank bioreactors. We've built an in-house capacity with over 80 bioreactors ranging from 2L to 300L culture volume. This large capacity can support both mouse hybridoma cell culture and transient transfection of 293 cells for recombinant mouse antibody production.

How to Submit a Request for Antibody Production Service?

If you are interested in using our antibody production services to support your antibody discovery and development program, please contact us by leaving an on-line message or send us your detailed request to CRO-service@sinobiological.com. Based on the quantity and quality requirement of your request, a formal quote will be send back to you for your review, comment, and/or acceptance.

Biology CRO Service
Biology CRO Service Introduction
Antibody Production & Development CRO Service
Antibody /Mab Production CRO Service+
- Antibody Production Service Description
- Ab Production Service Q&A
- Antibody /Mab Production Service: mini-scale (0.1-1 mg)
- Antibody /Mab Production Service: Small-scale (1-10 mg)
- Antibody /Mab Production Service: Mid-scale (10-100 mg)
- Antibody /Mab Production Service: Large-scale (100 mg - 1 gram)
- Transient Transfection Antibody Production: Super-scale (1g - 100 gram)
- Stable CHO Antibody Production Service: Super-scale (1g - 100 gram)
- Single-Chain ScFv Antibody Fragment Production Service
- Antibody / Mab Fab production Service
- Mouse Mab production CRO Service
Mouse Mab Production Description
mouse Mab production from hybridoma cell culture
recombinant mouse Mab production service
- Rat antibody production CRO service
- Rabbit Mab production CRO Service
Protein Production CRO Service
Transient Transfection Service: Mammalian Cell Culture
Molecular Biology CRO Service