Myc-tag Protein Expression, Production, and Purification Basics

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What is a Myc-tag?

A Myc-tag is a polypeptide protein tag derived from the c-myc gene product that can be fused to either the C- or N-terminus of a protein through recombinant DNA technology. It can facilitate the affinity purification of the tagged protein, as well as the isolation and detection of the tagged protein.

The peptide sequence of the myc-tag is: EQKLISEEDL (or -Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-).

What is the Molecular Wieght of the Myc-tag?

The Myc-tag contains 10 amino acids, and the molecular weight of the Myc-tag (EQKLISEEDL-tag) peptide is 1202 Da.

Why use a Myc-tag for protein expression and production?

Adding a Myc-tag to a protein's C- or N-terminus can facilitate its detection, isolation, and purification by Myc-tag specific antibodies. This is particularly helpful for proteins without a detection antibody or with unknown cellular location. Sino Biological has developed Myc-tag specific antibodies for ELISA, Western Blotting, Immonofluorecence, and Immunoprecipitation detection of the Myc-tagged protein. It can also be conjugated to agarose beads for Myc-tagged protein affinity purification.

How to Purify Myc-tagged Proteins?

Myc-tagged recombinant protein can be affinity purified directly from a cell culture lysate or supernatant. The Myc-tagged protein binds to the Myc-tag specific monoclonal antibody conjugated on an agarose gel. After washing away residual impurities, bound Myc-tag proteins can be eluted off the affnity column by high concentration of the Myc-tag peptide or by low pH buffer. For more information on usage of the Myc-tag affinity purification resin, please refer to: Myc-tag Affinity Resin.

How do I cleave of the Myc-tag after purification?

In some applications, it is desirable to remove the Myc-tag, for example, for protein crystalization. To allow cleavage of the Myc-tag, a protease cleavage site needs to be engineered between the tag and the protein. An EK cleavage site behind the Myc-tag (Myc-EK site-protein structure) can allow complete removal of the Myc-tag and the cleavage site, leaving no additional amino acids after the specific cleavage of the Myc-tag. For more information on the cleavage site and tag removal by EK and HRV-3C protease, please refer to: Enterokinase (EK), HRV-3C (human rhinovirus protease).

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