acnB Matched ELISA Antibody Pair Set,E. coli
For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.
acnB Matched ELISA Antibody Pair Set,E. coli 产品信息
产品名称
acnB Matched ELISA Antibody Pair Set,E. coli
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of E. coli acnB
线性范围
54.69-3500 pg/mL
ELISA 抗体对套装（非即用型）组分
1. Capture Antibody 1 mg/mL of mouse anti-Escherichia coli acnB monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 1 μg/mL in PBS before coating. (Catalog: # 13102-MM09)
2. Detection Antibody 0.2 mg/mL of mouse anti-Escherichia coli acnB monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 13102-MM02)
3. Standard Each vial contains 214 ng of recombinant Escherichia coli acnB. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3500 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of mouse anti-Escherichia coli acnB monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 13102-MM02)
3. Standard Each vial contains 214 ng of recombinant Escherichia coli acnB. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3500 pg/mL is recommended.
产品概述
This acnB Matched ELISA Antibody Pair Set,E. coli is a solid phase sandwich ELISA for quantitative determination of E. coli acnB . It contains E. coli acnB capture antibody, E. coli acnB detector antibody
(HRP)
and a highly purified
E. coli-expressed
recombinant E. coli acnB protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
acnB Matched ELISA Antibody Pair Set,E. coli 图片
acnB Matched ELISA Antibody Pair Set,E. coli: 别称
yacI Matched ELISA Antibody Pair Set, E. coli; yacJ Matched ELISA Antibody Pair Set, E. coli
acnB 背景信息
Escherichia coli contains two major aconitases (Acns), AcnA and AcnB. They are distantly related monomeric Fe-S proteins that contain different arrangements of four structural domains. acnA is specifically subject to SoxRS-mediated activation, whereas acnB encodes the major aconitase that is synthesized earlier in the growth cycle than AcnA. It is concluded that AcnB is the major citric acid cycle enzyme. Aconitate hydratase 2 (acnB) catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate, thus it functions as the major citric-acid-cycle enzyme during exponential growth. Escherichia coli acnB serves as either an enzymic catalyst or a mRNA-binding post-transcriptional regulator, depending on the status of its iron sulfur cluster. AcnB represents a large, distinct group of Gram-negative bacterial aconitases that have an altered domain organization relative to mitochondrial aconitase and other aconitases.
参考文献
- Bradbury AJ. et al., 1996, 142 (Pt 2): 389-400.
- Cunningham L. et al., 1997, Microbiology. 143 (Pt 12): 3795-805.
- Jordan PA. et al., 1999, Biochem J. 344 Pt 3: 739-46.
- Williams CH. et al., 2002, Nat Struct Biol. 9(6): 447-52.
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