1. Capture Antibody 1 mg/mL of mouse anti-Escherichia coli acnB monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 1 μg/mL in PBS before coating. (Catalog: # 13102-MM09) 2. Detection Antibody 0.2 mg/mL of mouse anti-Escherichia coli acnB monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 13102-MM02) 3. Standard Each vial contains 214 ng of recombinant Escherichia coli acnB. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3500 pg/mL is recommended.
This acnB Matched ELISA Antibody Pair Set,E. coli is a solid phase sandwich ELISA for quantitative determination of E. coli acnB . It contains E. coli acnB capture antibody, E. coli acnB detector antibody
and a highly purified
recombinant E. coli acnB protein. This Pair Set is at affordable price for researchers.
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE! Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
acnB Matched ELISA Antibody Pair Set,E. coli 图片
acnB Matched ELISA Antibody Pair Set,E. coli: 别称
yacI Matched ELISA Antibody Pair Set, E. coli; yacJ Matched ELISA Antibody Pair Set, E. coli
Escherichia coli contains two major aconitases (Acns), AcnA and AcnB. They are distantly related monomeric Fe-S proteins that contain different arrangements of four structural domains. acnA is specifically subject to SoxRS-mediated activation, whereas acnB encodes the major aconitase that is synthesized earlier in the growth cycle than AcnA. It is concluded that AcnB is the major citric acid cycle enzyme. Aconitate hydratase 2 (acnB) catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate, thus it functions as the major citric-acid-cycle enzyme during exponential growth. Escherichia coli acnB serves as either an enzymic catalyst or a mRNA-binding post-transcriptional regulator, depending on the status of its iron sulfur cluster. AcnB represents a large, distinct group of Gram-negative bacterial aconitases that have an altered domain organization relative to mitochondrial aconitase and other aconitases.
Bradbury AJ. et al., 1996, 142 (Pt 2): 389-400.
Cunningham L. et al., 1997, Microbiology. 143 (Pt 12): 3795-805.
Jordan PA. et al., 1999, Biochem J. 344 Pt 3: 739-46.
Williams CH. et al., 2002, Nat Struct Biol. 9(6): 447-52.